Components

20 rxn40 rxnr> 2 × 0.5 µg4 × 0.5 µgBlunt Vectorr> 10 µl10 µlPositive Control Insertr> 100 µl2 × 100 µlEnd Conversion Mix

General description

The pSTBlue-1 vector is a multi-purpose cloning vector featuring a versatile multiple cloning region, blue/white screening, dual opposed T7/SP6 promoters and dual kanamycin/ampicillin resistance. Restriction sites producing 4-base 3′ overhangs are conveniently positioned near each end of the polylinker to facilitate the generation of unidirectional deletions using exonuclease III.For enhanced convenience, please consider Introductory pSTBlue-1 Perfectly Blunt^®Cloning Kit, or pT7Blue^™-1 Perfectly Blunt Cloning Kit.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

PT7BLUE is a trademark of EMD Chemicals Inc.

Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Novy, R.E., et al. 1996. inNovations6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques20, 1004. Magnuson, V.L., et al. 1996. BioTechniques21, 700. Hu, G. 1993. DNA and Cell Biology12, 763.

Packaging

20 reactions in Plastic ampoule

40 reactions in Glass bottle

Warning

Toxicity: Standard Handling (A)